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University of Nebraska–Lincoln

AllergenOnline

Home of the farrp allergen protein database

Latest News:

New Version
Version # 18B
Peer Reviewed Sequences 2089
taxonomic-protein groups 831
Released On March 23, 2018
Download: V18B.pdf

Welcome to AllergenOnline.org

AllergenOnline provides access to a peer reviewed allergen list and sequence searchable database intended for the identification of proteins that may present a potential risk of allergenic cross-reactivity. This website was designed to help in assessing the safety of proteins that may be introduced into foods through genetic engineering or through food processing methods. The objective is to identify proteins that may require additional tests, such as serum IgE binding, basophil histamine release or in vivo challenge to evaluate potential cross-reactivity.

The database is updated annually. Version 4 was released on a public website in 2004. Version 18B was released on 23 March 2018 as there were a few errors as explained on the History Page. The database is freely accessible with the intent of providing a simple and useful tool that may be useful in food safety evaluations.

NEW on 10 May 2018: we have added information for each entry that states whether evidence was found indicating IgE binding as well as biological activity of basophil activation or skin prick test positivity for individual entries, or if the protein was only shown to have in vitro IgE binding. That information is available on the Browse page and it is intended to aid in a more accurate evaluation of protein risk, since there are tremendous differences in risk to different "allergens". It is important to note that proteins that do not have proof of biological activity may indeed be important allergens, but they have not been fully tested. Likewise, proteins with positive bioactivity may have been tested at very high doses. Review of references associated with the allergens are necessary to understand risk. Dose is also an important factor to consider.

Features and Tools Available.

Sequence search routines for food safety

  • We continue provide simple amino acid search routines to allow you to compare a protein sequence with the sequences in the current AllergenOnline database, which is updated on an annual basis. This is intended primarily for evaluating new proteins in Genetically Modified crops or in Novel Foods.
  • Search for full-length alignments by FASTA: The most predictive search is the overall FASTA alignment (see FASTA Help Page), with identity matches greater than 50% indicating possible cross-reactivity (Aalberse, 2000).
  • Search for 80 amino acid alignments by FASTA: A precautionary search using a sliding window of 80 amino acid segments of each protein to find identities greater than 35% (according to CODEX Alimentarius guidelines, 2003).
  • Search for 8 amino acid exact match: An 8-amino acid short-sequence identity search is provided since some regulatory authorities demand results of this extremely precautionary search. Our scientific opinion is that there is no evidence that an 8 amino acid match will identify a protein that is likely to be cross-reactive and could be missed by the conservative 80 amino acid match (35%). In our experience, isolated identity matches of 8 contiguous amino acids occur by chance alone at some modest rate, matches of 7 and 6 occur more commonly.  Experience (published and unpublished) demonstrates that two proteins sharing only a single short identity match of from 6 to 8 contiguous amino acids do not share IgE binding in the absence of more extensive identity alignments (at least >35% identity over 80 or more amino acids).  And that sequences sharing less than 50% identity over their full-lengths are rarely cross-reactive.  Thus we recommend not using these short identity matches as there is no scientific evidence that they predict IgE cross-reactivity and they do not predict shared clinical activities.

Browse the database

  • Allergen Source: Sequences of allergens are compiled in a table under "species", (scientific name: genus and species) and common name of the source.
  • Each sequence is listed separately:  There can be multiple isoforms or partial sequences for a single allergen (e.g. Actinidia deliciosa Act d 1)
  • WHO/IUIS  Allergen Nomenclature: (World Health Organization/International Union of Immunological Societies) designation or name is shown if known abbreviated as IUIS.
  • Type of allergen is shown:  (e.g. Aero plant, food animal, venom or salivary)
  • Allergen Groups: Group names include the genus and protein type or IUIS designation.  Multiple isoforms or close homologues of proteins contained in the database were clustered based on taxonomy of the source organism and sequence identity of the proteins. Evidence of allergenicity is assembled and evaluated for the collective sequences within one group, for review by the AllergenOnline.org expert panel and for presentation of references related to the sequences.  The allergen “group” is linked to more information including the published references describing the information used to classify the protein as an allergen, as well as the individual sequences clustered into the group.  The Accession# and GI# in the table of allergens are linked to the NCBI entries to show the complete NCBI entry, although GI#s are no-longer shown in NCBI. Publication references are listed in the Group or gid list.  References may apply to a single sequence, or multiple sequences in the group. They provide evidence of the allergenicity of the source.
  • References: The group name links to "Group References" describing the evidence of allergenicity for the group and a list of "Group Sequences" included in this version of the database.
  • Length:  The number of amino acids is shown for each allergen entry
  • Accession # and GI#:  The NCBI record number is linked directly to NCBI for viewing.
  • Version:  The database version that the specific allergen was entered is included (e.g. 7, 9, 11, 18)

Celiac disease protein Database Risk Assessment Tool

in 2012 FARRP added a new bioinformatics tool in response to the CODEX Alimentarius Guidelines approved in 2003, to compare query sequences to peptides and proteins known to elicit celiac disease in some people. The database is entered as a separate link on the left side of this page. It was updated to version 2 in January, 2018 and now includes 1013 peptides, 72 proteins and 72 references. This tool meets the new European Food Safety Authority guideline for evaluating proteins from wheat and wheat-relatives for risks of celiac disease (June, 2017). A manusript is in preparation describing the methods, the comparisons that include identity matches to peptides, or high scoring FASTA3 alignments to any of the 72 proteins. The database provides an exact peptide query and FASTA3 search algorithms to evaluate whether a novel protein derived from wheat.

Who We Are

Allergen Online Team

This database was developed and is maintained by the Food Allergy Research and Resource Program (FARRP) in the Department of Food Science and Technology at the University of Nebraska in Lincoln. John Wise wrote the code and maintained the database through version 14. Sreedevi Lalithambika joined the database in 2014 to 2016 to maintain the system and helping identify new candidate allergens. Richard Goodman manages the database and leads the peer review team.

Peer Reviewers

A panel of scientists and clinicians are actively involved in reviewing data for inclusion of proteins in the database by comparing peer reviewed publications supporting the classification of the proteins as allergens or putative allergens following predetermined guidelines. The affiliations of the experts are listed below. However, the experts participate as independent consultants. There is no claim that the opinions of the experts or the information provided in this allergen website, has been endorsed by any of their institutions.

  • Joe Baumert, Ph.D.,
    University of Nebraska, Lincoln, NE, USA
  • Barbara Bohle, Ph.D.,
    Medical University of Vienna, Vienna, Austria
  • Motohiro Ebisawa, M.D.,
    National Sagamihara Hospital, Sagamihara, Japan
  • Fatima Ferreira, Ph.D.,
    University of Salzburg, Salzburg, Austria
  • Richard E. Goodman, Ph.D.,
    University of Nebraska, Lincoln, NE, USA
  • Joerg Kleine-Tebbe, MD, FAAAAI., (member since 2016)
    Allergie- & Asthma-Zentrum Berlin Westend, OPD Hanf, Ackermann & Kleine-Tebbe, Berlin, Germany
  • Steve L. Taylor, Ph.D.,
    University of Nebraska, Lincoln, NE, USA
  • Ronald van Ree, Ph.D.,
    Academic Medical Center, Amsterdam, The Netherlands
Former members:
  • Sue L. Hefle, Ph.D., (2005-2006)
    University of Nebraska, Lincoln, NE, USA
  • Stefan Vieths, Ph.D., (2005-2012)
    Paul-Ehrlich-Institut, Langen, Germany
  • Hugh A. Sampson, M.D., (2005-2016)
    Mount Sinai Medical Center, New York, NY, USA

Financial Support

Financial support for this database has been provided by grants from corporate subscribers and by FARRP (the Food Allergy Research and Resource Program, Department of Food Science & Technology at the University of Nebraska-Lincoln) and faculty. Support in 2018 and beyond is primarily from faculty.

The majority of the scientific information for the Allergen database and now for the Celiac database is collected and evaluated by Rick Goodman, then verified by the review team. The database is updated annually. The database construction was performed by John Wise, who now consults to help maintain it.

Sponsors (2004 - 2015):
  • BASF Plant Science, LP
  • Bayer CropScience, AG
  • Dow AgroSciences, LLC
  • KWS SAAT AG
  • Monsanto Company
  • Pioneer Hi-Bred International, Inc., DuPont
  • Syngenta Crop Protection, LLC
  • Vilmorin & Cie

Subscribers (2016 - 2017):

  • BASF Plant Science, LP
  • Pioneer Hi-Bred International, Inc., DuPont
  • J.R. Simplot Company
  • Nuseed
  • Unilever
  • <

Subscribers 2018:

  • Nuseed
  • Unilever
  •  

    Liability statement

    The content of the AllergenOnline.org database is the sole responsibility of researchers at the University of Nebraska-Lincoln, with the advice and collaboration of additional independent academic and medical consultants. This work is or was co-sponsored by the aforementioned corporate subscribers and the Food Allergy Research and Resource Program at the University of Nebraska-Lincoln. Corporate subscribers had the right to provide scientific input as suggestions. However, all decisions regarding content are the responsibility of the FARRP expert panel. The subscribers and the University of Nebraska, make no representations or warranties of any kind, express or implied, concerning the content of the database, including, without limitation, warranties of merchantability, fitness for a particular purpose, non-infringement, validity of any intellectual property rights or claims, whether issued or pending, and the absence of latent or other defects, whether or not discoverable.

    Bioinformatic Allergen Assessment Reports

  • Siruguri V, Bharatraj DK, Vankudavath RN, Rao Mendu VV, Gupta V, Goodman RE. Evaluation of Bar, Barnase, and Barstar recombinant proteins expressed in genetically engineered Brassica juncea (Indian mustard) for potential risks of food allergy using bioinformatics and literature searches. Food Chem Toxicol. 2015 Jun 14;83:93-102. doi: 10.1016/j.fct.2015.06.003. [Epub ahead of print] PubMed PMID: 26079618.

  • Jin Y, Goodman RE, Tetteh AO, Lu M, Tripathi L. Bioinformatics analysis to assess potential risks of allergenicity and toxicity of HRAP and PFLP proteins in genetically modified bananas resistant to Xanthomonas wilt disease. Food Chem Toxicol. 2017; 109:81-89. http://doi.org/10.1016/j.fct.2017.08.024 (Open Access, PMID: 28830835).
  • Jin Y, He X, Ando-Kumi K, Fraser RZ, Lu M, Goodman RE. Evaluating potential risks of food allergy and toxicity of soy leghemoglobin expressed in Pichia pastoris. Mol Nutr Food Res. 2017 Sept. 18; doi: 10.1002/mnfr.201700297 [Epub ahead of print](Open Access, PMID: 28921896).